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Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP11A(E186Q)-HA
Plasmid#209249PurposeMammalian expression of ATP11A mutant E186QDepositorInsertATP11A (ATP11A Human)
UseTagsHAExpressionMammalianMutationE186QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaMBI_P2A_Hygro_Barcode
Plasmid#120504PurposeBarcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaMBI (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of MYC Box I domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaMBII_P2A_Hygro_Barcode
Plasmid#120505PurposeBarcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertc-MYC deltaMBII (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of MYC Box II domainPromoterEF1aAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNLS_P2A_Hygro_Barcode
Plasmid#120506PurposeBarcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC H84NLS (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of nuclear localization signal sequencePromoterEF1aAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd
Plasmid#193835PurposeExpress TadCBEd (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-RdLight1
Plasmid#125708PurposeExpresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.DepositorHas ServiceAAV9InsertRdLight1
UseAAVTagsFlag tagExpressionMutationPromoterhSynapsin1Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RdLight1
Plasmid#125707PurposeExpresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.DepositorHas ServiceAAV9InsertdLight1.3b
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB556
Plasmid#115966PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/15 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-R203M
Plasmid#177952PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (R203M) (N )
UseTagsExpressionMammalianMutationR203MPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-hEPO
Plasmid#50436Purposeexpress human erythropoietin using lentiviral delivery systemDepositorInsertEPO (EPO Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CLXSN 5myc-CDC6-WT
Plasmid#109332Purposeexpress tagged Cdc6 WTDepositorInsertCDC6 (CDC6 Human)
UseRetroviralTags5x MycExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSE401
Plasmid#62202PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only