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Plasmid#203168PurposeYeast expression vector for human DHDDS K42EDepositorInsertDHDDS (DHDDS Human)
UseTagsExpressionYeastMutationK42EPromoterTDH3Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-hCIT
Plasmid#203166PurposeYeast expression vector for human DHDDSDepositorInsertDHDDS (DHDDS Human)
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-UBQLN2
Plasmid#181708PurposeGalactose inducible expression of UBQLN2DepositorInsertUBQLN2 (UBQLN2 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-atTIR1-9myc
Plasmid#99532PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorInsertTIR1 (TIR1 Mustard Weed)
UseTags9xMycExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationSee manuscriptPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-DENV_NS3-GS-NS2B
Plasmid#201940PurposeExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertDENV NS3-GS-NS2B (POLY )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-WNV_NS2B-GS-NS3
Plasmid#203475PurposeExpresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 markerDepositorInsertWNV NS2B-GS-NS3 (POLY )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pINO4-Pfv-Sapphire-HO
Plasmid#203659PurposeYeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus.DepositorArticleInsertPfV
UseCRISPRTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Mms2
Plasmid#18893DepositorInsertYeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
UseTagsHIS10ExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53206PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and TRP1ExpressionMutationPromoterGAL1 promoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53205PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and kanMX6ExpressionMutationPromoterGal1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGPDluxAB(Ura)
Plasmid#1119DepositorInsertbacterial luciferase
UseTagsExpressionYeastMutationn/aPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53207PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and HIS3MX6ExpressionMutationPromoterGAL1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEP1Hsp82NdeI
Plasmid#1276DepositorInsertHsp82 (HSP82 N/A)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJJB1561
Plasmid#218626PurposeExpress geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1439
Plasmid#218589PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniolDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W)PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB170
Plasmid#218591PurposeExpress engineered TFs Adr1c(S230A), Pip2c, and Oaf1cDepositorInsertAdr1
UseTagsExpressionYeastMutationAdr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKT001
Plasmid#218594PurposeExpress degron assay: Degradation tag-YFP-ePTS1DepositorInsertYFP
UseTagsUbiY degradation tag and ePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only