Addgene

We narrowed to 6,974 results for: chloramphenicol

Showing: 6181 - 6200 of 6974 results

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pnYC-NvHN-DmXRN1_1323-1355_N

Plasmid

#147052

Purpose

Bacterial Expression of DmXRN1_1323-1355

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355 (pcm Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-udhA-xylA

Plasmid

#158612

Purpose

Low phosphate inducible gRNA array to silence the xylA and udhA promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

udhA-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA2-xylA

Plasmid

#158613

Purpose

Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

gltA2-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-zwf-xylA

Plasmid

#158614

Purpose

Low phosphate inducible gRNA array to silence the xylA and zwf promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

zwf-xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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CDS12-MpER-Targ

Plasmid

#136094

Purpose

CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

ER targeting peptide from predicted chitinase Mapoly0069s0092

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_eGFP-GHtag-HDEL

Plasmid

#136096

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUAP12008

Plasmid

#177036

Purpose

Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

Cauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0065

Plasmid

#177019

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase

Use

Synthetic Biology

Tags

Expression

Mutation

transit peptide removed, amplified from cDNA, con…

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pTF101.1gw3

Plasmid

#172181

Purpose

A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Paz et al Plant Cell Rep. 2006 Mar;25(3):206-13. d

Insert

attR3-CmR-ccdB-attR4

Use

Tags

Expression

Plant

Mutation

Promoter

lacUV5 promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtU6-26:AsCas12a_DR (GB1442)

Plasmid

#160574

Purpose

AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtU6-26:AsCas12a_DR

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 LbCas12a (GB1439)

Plasmid

#160573

Purpose

Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

LbCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AsCas12a (GB1438)

Plasmid

#160572

Purpose

Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AsCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-DAB1

Plasmid

#162691

Purpose

Expresses DabAB1 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB1 inorganic carbon transport complex

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-9251

Plasmid

#124226

Purpose

Bacterial SpCas9-HF1 expression

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

SpCas9-HF1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

TetR/TetA

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 TNos:Luc:5'UTR (GB1499)

Plasmid

#160580

Purpose

Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 linker-RDF (GB2892)

Plasmid

#160604

Purpose

RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MTK234_058

Plasmid

#123984

Purpose

Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sg Safetargeting Chr11

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_038

Plasmid

#123917

Purpose

Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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