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Plasmid#59825PurposeAllows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55/S1126APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-HA-Non-stop
Plasmid#52289PurposeExpresses Drosophila Non-stopDepositorInsertNon-stop (not Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB9 (murine) HIS-tag pET
Plasmid#8541DepositorInsertHOXB9 (Hoxb9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationMissing the first 13 amino acids of HOXB9; makes …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
sgGCAT_2
Plasmid#72885PurposeCRISPR-Cas9 induced gene disruptionDepositorInsertsgGCAT
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMGF170
Plasmid#96997Purposeish1-mCherry insertion cassette with NAT resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + NAT r…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-221SM-PGK-GFP-Blast
Plasmid#31520DepositorInsertmiR-221 (Mir221 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-196a-1SM-PGK-GFP-Blast
Plasmid#31510DepositorInsertmiR-196a1 (Mir196a-1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Hoxa4-3'UTR
Plasmid#31524DepositorInsertHoxa4-3'UTR (Hoxa4 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T35A
Plasmid#47937Purposeexpresses Myc tagged human MOB1 containing T35A mutationDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT35APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-324SM-PGK-GFP-Blast
Plasmid#31518DepositorInsertmiR-324 (Mir324 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
3704 pGEX4T-3 MLL AD L2854A
Plasmid#8876DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationL2854A: ILPSDIMDFVL --> ILPSDIMDFVAPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-EYFP
Plasmid#231927PurposeCre-off/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS
Plasmid#228400PurposeEncodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasmDepositorInsertNES/PKI-mNeonGreen2x-NLS/FUS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro
Plasmid#228398PurposeEncodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasmDepositorInsertNLS/cMyc-AcGFP2x-NES/iKB2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro
Plasmid#228399PurposeEncodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasmDepositorInsert3xFLAG-NLS/SV40-AcGFP-NES/PKI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
MLF2-GFP-3xFLAG
Plasmid#230986PurposeExpresses GFP tagged MLF2 in mammalian cellsDepositorInsertMLF2 (MLF2 Human)
UseTags3xFLAG and GFPExpressionMammalianMutationPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:FRB:gs10:Cul1(dC501)
Plasmid#228533PurposeA fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWT-IFl_WT-IFr
Plasmid#192207PurposeWildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertWildtype I-F Leader and wildtype I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only