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Plasmid#130936PurposePiggyBac vector for constitutive NanoLuc expression in mammalian cellsDepositorInsertNanoLuc
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
vYM140_PB-pEFa1-iaaM(P.s)-Hygro
Plasmid#160043PurposeExpressing iaaM for converting L-Trp to IAMDepositorInsertiaaM (iaaM )
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpEF1aAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
vYM112_PB-pEF1a-optiPIN2-HA(Hygro)
Plasmid#160045PurposeFor expressing the active auxin pump PIN2DepositorInsertPIN2
UseSynthetic BiologyTagsHAExpressionMammalianMutationcodon optimizedPromoterpEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.139
Plasmid#184994PurposeExpress Cas9-P2A-Eco1RT in human cellsDepositorInsertSpCas9-P2A-Eco1RT
UseTagsSV40NLSExpressionMammalianMutationHuman codon optimized RTPromoterTetOn-3gAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
Plasmid#160042PurposeFor the auxin receiver that report on auxin.DepositorInsertsosTIR1
BlastR
UseLuciferaseTagsmAID and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-WT(21)
Plasmid#124396PurposeFull-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 (TET1 Human)
UseTagsFlagExpressionMammalianMutationfull length protein is 2136 aaPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2
Plasmid#175499PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
KA0637_pPBCAG-rtTAM2-IN
Plasmid#124166PurposepiggyBac-based expression of rtTA-M2DepositorInsertrtTAM2-IRES-Neo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg30-PDGFR_EF1a_mCherry
Plasmid#186309PurposePlasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg30-PDGFR
mCherry
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2
Plasmid#175497PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo
Plasmid#167901PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
vas-Cas9.874Z
Plasmid#112687PurposeExpress hSpCas9 under vasa promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromotervasaAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-T3G-ERP2
Plasmid#175188PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.16_PB-Cx43_S368A-PGK-Blast
Plasmid#205561PurposeExpress human Cx43 with S368A mutationDepositorInsertCx43
UsePiggybacTagsExpressionMammalianMutationS368APromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only