Addgene

We narrowed to 7,192 results for: pCas

Showing: 601 - 620 of 7192 results

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pSpCas9(BB)-2A-Puro-SMCR8

Plasmid

#83440

Purpose

Expresses Cas9 and a gRNA targeting SMCR8

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human SMCR8 (SMCR8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-C9orf72

Plasmid

#83439

Purpose

Expresses Cas9 and a gRNA targeting C9orf72

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human C9orf72 (C9orf72 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA1-gltA2

Plasmid

#71348

Purpose

gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

gltA1-gltA2 guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ePsuACR-mRuby2-ST

Plasmid

#109133

Purpose

Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ePsuACR-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-eiC++-mRuby2-ST

Plasmid

#109132

Purpose

Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eiC++-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-CsChR-mRuby2-ST

Plasmid

#109130

Purpose

Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CsChR-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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zwf-fbp-opcA-

Plasmid

#101839

Purpose

deletes zwf_fbp_opcA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_2333-2335 operon)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCASCADE-udhA-zwf

Plasmid

#87153

Purpose

udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

udhA-zwf guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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CpcB-Pinus-banksianaPHLS_CpcA

Plasmid

#74002

Purpose

Expresses the Pinus banksiana PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CpcB-Picea-sitchensisPHLS_CpcA

Plasmid

#74001

Purpose

Expresses the Picea sitchensis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CpcB-Abies-grandisPHLS_CpcA

Plasmid

#74000

Purpose

Expresses the Abies grandis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Insert

PHLS and CmR

Use

Tags

Expression

Mutation

Promoter

cpc

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA2-udhA

Plasmid

#65819

Purpose

gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Insert

gltA2-udhA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induc…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCaShs-egghead (zw4)

Plasmid

#37400

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Plasmid

#101215

Purpose

Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Depositor

Keith Joung, Jennifer Doudna

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K848A/K1003A/R1060A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K848A, K1003A and R1060A

Promoter

T7

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

Plasmid

#208976

Purpose

Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

nSpCas9(H840)-BPNLS-P2A-GFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCA-mTmG

Plasmid

#26123

Depositor

Liqun Luo

Insert

Precursor mT/mG

Use

Tags

membrane tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-CmR(+)

Plasmid

#113633

Purpose

Chloramphenicol resistant derivative of pCas plasmid

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

Chloramphenicol resistance gene

Use

CRISPR

Tags

Expression

Mutation

Promoter

Pcat

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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