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Plasmid#226263PurposePlasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 geneDepositorInsertLEU2 gRNA (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMVN
Plasmid#223861Purpose4G-cloning donor for mammalian promoter and terminator, N-terminal tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_phN
Plasmid#223858Purpose4G-cloning donor for insect promoter and terminator, N-terminal tag addedDepositorInsertpolyhedrin promoter and SV40 polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-TS-His10
Plasmid#223893Purpose4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tagDepositorInsert3C-TwinStrep-His10
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_Avi-3C-TS
Plasmid#223894Purpose4G-cloning donor for C-terminal AviTag-3C-TwinStrep tagDepositorInsertAviTag-3C-TwinStrep
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-GST-TEV
Plasmid#223878Purpose4G-cloning donor for N-terminal His6-GST-TEV tagDepositorInsertHis6-GST-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-MBP-TEV
Plasmid#223877Purpose4G-cloning donor for N-terminal His6-MBP-TEV tagDepositorInsertHis6-MBP-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-HA2-TEV
Plasmid#223876Purpose4G-cloning donor for N-terminal His6-HA2-TEV tagDepositorInsertHis6-HA2-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3-TEV
Plasmid#223875Purpose4G-cloning donor for N-terminal His6-FLAG3-TEV tagDepositorInsertHis6-FLAG3-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_phC
Plasmid#223859Purpose4G-cloning donor for insect promoter and terminator, C-terminal tag addedDepositorInsertpolyhedrin promoter and SV40 polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_T7N
Plasmid#223855Purpose4G-cloning donor for bacterial promoter and terminator, N-terminal tag addedDepositorInsertT7 promoter and terminator
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_T7C
Plasmid#223856Purpose4G-cloning donor for bacterial promoter and terminator, C-terminal tag addedDepositorInsertT7 promoter and terminator
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His10-TS-3C
Plasmid#223884Purpose4G-cloning donor for N-terminal His10-TwinStrep-3C tagDepositorInsertHis10-TwinStrep-3C
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-Venus-His8
Plasmid#223889Purpose4G-cloning donor for C-terminal 3C-Venus-His8 tagDepositorInsert3C-Venus-His8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMVC
Plasmid#223862Purpose4G-cloning donor for mammalian promoter and terminator, C-terminal tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iPASnFR-L
Plasmid#225238PurposeBacterial expression of iPASnFR-LDepositorInsertiPASnFR-L
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iPASnFR-H
Plasmid#225237PurposeBacterial expression of iPASnFR-HDepositorInsertiPASnFR-H
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-jRGECO1a
Plasmid#210399PurposeAAV-vector for fluorescent calcium measurements in the red spectrum from noradrenergic neuronsDepositorInsertjRGeco1a
UseAAVTagsExpressionMammalianMutationPromoterPRSx8Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-GCaMP8m
Plasmid#210400PurposeAAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neuronsDepositorInsertGCaMP8m
UseAAVTagsExpressionMammalianMutationPromoterPRSx8Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA036
Plasmid#200425PurposeamyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsbsdronPAExpressionBacterialMutationPromoterPpenAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only