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Plasmid#71558PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationdeletes alanine-367 (same as deleting alanine-368)PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK624
Plasmid#72539PurposeProduces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK625
Plasmid#72541PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK392
Plasmid#70236PurposeProtein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40NDepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationHis40AsnPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD432
Plasmid#63755PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.DepositorInsertEAggEC
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-EGFP-PRC1
Plasmid#167540PurposeFor expression of His6-TEV-EGFP-PRC1 in bacteria.DepositorInsertPRC1 (PRC1 Human)
UseTagsEGFP and His6 tagExpressionBacterialMutationPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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His-TEV-SNAP-PRC1
Plasmid#167541PurposeFor expression of His6-TEV-SNAP-PRC1 in bacteria.DepositorInsertPRC1 (PRC1 Human)
UseTagsHis6 tag and SNAP tagExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro IkB alpha M
Plasmid#12332DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP5
Plasmid#178071PurposeInsect cell expression of GCP5DepositorInsertGCP5 (TUBGCP5 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK225
Plasmid#71124PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coliDepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK220
Plasmid#71123PurposeExpression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coliDepositorInsertpantothenate kinase
UseTagsH6ExpressionBacterialMutationArg175 is mutated to a HisPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK593
Plasmid#71587PurposeProduces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE )
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK599
Plasmid#72524PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK618
Plasmid#72535PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK594
Plasmid#71588PurposeProduces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with C-terminal His6 tag (YfdEH6)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE )
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK349
Plasmid#71566PurposeProduces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK272
Plasmid#71562PurposeProduces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK227
Plasmid#71126PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
UseTagsGyrA inteinExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK226
Plasmid#71125PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only