Addgene

We narrowed to 740 results for: KO

Showing: 601 - 620 of 740 results

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EcNGRfE

Bacterial Strain

#48935

Purpose

Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)

Depositor

George Church

Bacterial Resistance

Spectinomycin

Available since

Dec. 3, 2014

Availability

Academic Institutions and Nonprofits only
Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Donor_ANKRD1_KO_Puro

Plasmid

#186667

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Puromycin (ANKRD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GPX8 rescue PLAK1

Plasmid

#161517

Purpose

Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

glutathione peroxidase 8 (GPX8 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Hygro

Plasmid

#186668

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Hygromycin (ANKRD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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EcNGRfEfC

Bacterial Strain

#48937

Purpose

Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)

Depositor

George Church

Bacterial Resistance

Spectinomycin

Available since

Dec. 3, 2014

Availability

Academic Institutions and Nonprofits only
lentiCRISPR v2-sgBAP1-1

Plasmid

#125837

Purpose

KO BAP1 gene

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAP1-2

Plasmid

#125838

Purpose

KO BAP1 gene

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_2

Plasmid

#195131

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 2 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_1

Plasmid

#195130

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 1 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pETCON-IgG1/design_50

Plasmid

#30301

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB20/design_70

Plasmid

#30254

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB20

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 10, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB28/design_25

Plasmid

#30260

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB28

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-E43/design_55

Plasmid

#30237

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

Mtb ACP2 designed binding protein E43

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB14/design_53

Plasmid

#30249

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB14

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB50/design_43

Plasmid

#30270

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB50

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB56/design_34

Plasmid

#30276

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB56

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB4/design_2

Plasmid

#30271

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB4

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 16, 2011

Availability

Academic Institutions and Nonprofits only