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Showing: 6141 - 6160 of 11193 results

  1. PB-GG-OMKSL-PGK-Puro

    Plasmid
    #102894
    Purpose
    PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102894-plasmid-map-sequence-id-198285

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  2. GG-EBNA-OMKSL-PP

    Plasmid
    #102899
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102899-plasmid-map-sequence-id-198366

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  13. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Article
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Use
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63726-plasmid-map-sequence-id-438433

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  14. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  15. pMasterK

    Plasmid
    #165081
    Purpose
    Episomal expression of reprogramming factors.
    Depositor
    Hideyuki Okano
    Article
    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    Use
    Episomal
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    165081-plasmid-map-sequence-id-444338

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  16. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
    Use
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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  18. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  19. pJH122

    Plasmid
    #68377
    Purpose
    pJH110 variant Hygromycin
    Depositor
    Trevor Charles
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    68377-plasmid-map-sequence-id-119860

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  20. T4 Lysozyme Q105E

    Plasmid
    #18501
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Q105E
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only
Showing: 6141 - 6160 of 11193 results