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pLEX_970_puro_DEST_V5_BFP

Plasmid

#45297

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD8 Flag

Plasmid

#74900

Purpose

Express COMMD8 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD8 (COMMD8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 RARalpha

Plasmid

#35557

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pNEXdelta-EGFP-ApAF

Plasmid

#13335

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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XE49 pt Beta-catenin-myc

Plasmid

#16840

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

pt beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Expression

Mutation

four point mutations introduced into the GSK-3 ph…

Promoter

Available since

June 25, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA6-LRRC55-V5-Flag-His

Plasmid

#127819

Purpose

Expression of human LRRC55 in mammalian cells

Depositor

Jiusheng Yan

Article

Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

Insert

LRRC55 (LRRC55 Human)

Use

Tags

V5, FLAG, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mCasp8

Plasmid

#208782

Purpose

Inducibly expresses mouse caspase8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mCaspase8 Cry2 (Casp8 Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

Plasmid

#185684

Purpose

Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Synthetic, Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

K121Y

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-E264A

Plasmid

#31730

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19. ():.

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Expression

Mutation

Changed E264 to Alanine

Promoter

None

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha WT

Plasmid

#128812

Purpose

To make virus expressing Doc2alpha WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta WT

Plasmid

#128814

Purpose

To make virus expressing Doc2beta WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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SF-U7snRNA-CypA-E4

Plasmid

#190695

Purpose

Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA antisense CypA Exon 4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U7 promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETa-mEos3.2-A69T

Plasmid

#98571

Purpose

photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mEos3.2-A69T

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO ALIX Bro1-V (1-702)

Plasmid

#80642

Purpose

Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

ALIX Bro1-V (PDCD6IP Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Residues 1-702

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

Plasmid

#158604

Purpose

Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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EYFP-IKB

Plasmid

#111194

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Brg1 Del2 pET28a

Plasmid

#122116

Purpose

Expression Brg1 amino acids 1-837 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Use

Tags

6XHis and His-T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

Plasmid

#127322

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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