We narrowed to 8,831 results for: sgRNA

1. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Expression

   Mammalian

   Mutation

   VQR

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p8267 LentiCRISPR v2 hygro sgNT-2

   Plasmid

   #193978

   Purpose

   Expression of spCas9 and non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9
   

   Use

   Lentiviral

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-sgCDKN1B#2 BFP

   Plasmid

   #60905

   Purpose

   A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB700 lenti gRNA EYFP

   Plasmid

   #167906

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. px458_Conc2

   Plasmid

   #134449

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

   Plasmid

   #232935

   Purpose

   Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   T2A-eGFP-SV40pA with Grb10 homology arms
   

   Use

   Mouse Targeting

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv2-Stuffer-HepmCherry

   Plasmid

   #192825

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_RAD21_1

   Plasmid

   #64057

   Purpose

   Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_CREB1_1

    Plasmid

    #64939

    Purpose

    Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM733

    Plasmid

    #68898

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. px458_Conc3

    Plasmid

    #134450

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMM764

    Plasmid

    #68901

    Purpose

    IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Mutation

    L51P

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700 lenti gRNA Blasto_zhang2.0

    Plasmid

    #167912

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p8389 LentiCRISPRv2 Neo sgNT-1

    Plasmid

    #221649

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDropVn

    Plasmid

    #184873

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pHpgRNA13

    Plasmid

    #160127

    Purpose

    To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-ADE2- HDV-TAMO
    

    Expression

    Yeast

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgDonor_lentiGuide

    Plasmid

    #184168

    Purpose

    LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Insert

    sgDonor
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Tags

    t2a mCherry

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. px459V2.0_Conc3

    Plasmid

    #134458

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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