We narrowed to 8,902 results for: sgRNA
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Plasmid#218187PurposeTo KO and genetrap mouse Vamp2 simultaneouslyDepositorInsertsgRNA (Vamp2 Mouse)
UseAAV and CRISPRAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-U6-Bamh1
Plasmid#203298PurposeFor expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-U6-Bgl11
Plasmid#203299PurposeFor expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-U6-Not1
Plasmid#203301PurposeFor expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-sfGFP
Plasmid#154306PurposeExpression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescenceDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA5-RFP
Plasmid#62251Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA5Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCut plasmid toolkit
Plasmid Kit#1000000118PurposeCas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.DepositorAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE Dicot Genome Editing Kit
Plasmid Kit#1000000084PurposeGolden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of "genome editing recipient" and "sgRNA shuttle" vectors.DepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCB320
Plasmid#89359Purpose3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sitesDepositorInsertpuromycin resistance, mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry
Plasmid#197420PurposeCas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNADepositorInsertCas9
UseCRISPRTagsmCherryExpressionMammalianAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeExpressionBacterialAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFi-dHdrA
Plasmid#184907Purposecarrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferriduransDepositorInsertCm-sgRNA-dCas9
UseCRISPRExpressionBacterialAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG79-attL1-pegRNA-attL2
Plasmid#213050PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
ExpressionBacterialPromoter35S-CmYCLV-AtU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRGEB32-BAR
Plasmid#126072PurposeFor CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoter35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…Available SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-gRNA-CBh-mCherry
Plasmid#91947PurposeExpression of empty gRNA cassette and mCherryDepositorInsertmCherry
UseAAVAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Opti
Plasmid#163126PurposeLentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas12f1
Plasmid#171182PurposeConstitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1DepositorInsertCas12f1
UseCRISPRExpressionBacterialPromotercas9 promoter from Streptococcus pyogenesAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Blasto
Plasmid#167904PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only