We narrowed to 8,845 results for: sgRNA

1. pICSL1P1_AtU626Ter26

   Plasmid

   #191769

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas12f1

   Plasmid

   #171182

   Purpose

   Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   Cas12f1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   cas9 promoter from Streptococcus pyogenes

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgRNA

   Plasmid

   #215560

   Purpose

   Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   gRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRGEB32-BAR

   Plasmid

   #126072

   Purpose

   For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

   Depositor

   Charles Hunter

   Article

   Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD5-NS

   Plasmid

   #149546

   Purpose

   Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVT36b

   Plasmid

   #184437

   Purpose

   CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

   Depositor

   Huimin Zhao

   Article

   Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRT2B-GO2

   Plasmid

   #136909

   Purpose

   Lentivirus expressing GO2 sgRNA

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   sgGO2
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   hU6

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAbaMCi-BsaI_pJMP2776

   Plasmid

   #208890

   Purpose

   Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8-attR4-pegRNA-attR3

   Plasmid

   #213056

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR8-attL5-pegRNA-attL4

    Plasmid

    #213055

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM704

    Plasmid

    #68891

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Expression

    Mammalian

    Mutation

    VQR

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB700 lenti gRNA mcherry

    Plasmid

    #167910

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p8267 LentiCRISPR v2 hygro sgNT-2

    Plasmid

    #193978

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB700 lenti gRNA EYFP

    Plasmid

    #167906

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. px458_Conc2

    Plasmid

    #134449

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPRv2-Stuffer-HepmCherry

    Plasmid

    #192825

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_RAD21_1

    Plasmid

    #64057

    Purpose

    Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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