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Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz33_CsoS2_NTD_GFP
Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
UseTagsHexahistidine tag and Superfolder GFPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz47_polyPro_CsoS2_Npeptide
Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
UseTagsHexahistidine tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer
Plasmid#138365PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence.DepositorInsert161k human enhancer
UseGateway compatibleTagsExpressionMutation9 A bases deletion, that have no impact on the en…PromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-Cerulean
Plasmid#127771PurposeEnhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activityDepositorInsertednrbE2
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE1-Citrine
Plasmid#127773PurposeEnhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activityDepositorInsertednrbE1
UseEnhancer reporter constructTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sox3gRNA-T2
Plasmid#127778PurposeChicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)DepositorInsertsox3gRNAT2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_006
Plasmid#123844PurposeEncodes spacer sequence (multicistronic) as a Type 4b part to be used in the MTK systemDepositorInsertSpacer for multicistronic
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4_006
Plasmid#123814PurposeEncodes spacer sequence (multicistronic) as a Type 4 part to be used in the MTK systemDepositorInsertSpacer for multicistronic
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-YbATP-grasp
Plasmid#129639PurposeExpression plasmid of ATP-grasp from Y. bercovieriDepositorInsertYbATP-grasp
UseTagsHis6-tagExpressionBacterialMutationPromoterpQEAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
2ERT2-AAVS1 TALEN-L
Plasmid#120544PurposeDrug inducible TALEN targeting the human AAVS1 locus for genome editingDepositorInsertERT2, hAAVS1 1L TALEN
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_110μ-pcDNA3
Plasmid#111929PurposeCyan color bright luminescent calcium indicator with low affinityDepositorInsertCeNL(Ca2+)_110μ
UseTagsExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ePsuACR-mRuby2-ST
Plasmid#109133PurposeAnion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInsertePsuACR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eiC++-mRuby2-ST
Plasmid#109132PurposeArtificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteiC++-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CsChR-mRuby2-ST
Plasmid#109130PurposeCation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertCsChR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)
Plasmid#108239PurposeExpresses PdR-PCNA2(L171C) fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C) fusion protein
UseTagsHis6 tagExpressionBacterialMutationPCNA2,L171CPromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T+1X
Plasmid#108188PurposePCNA1-PdX fusion proteinDepositorInsertPCNA1-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationC73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only