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Showing: 6081 - 6100 of 22037 results

  8. pFrt-invCAG-Luc

    Plasmid
    #63577
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63577-plasmid-map-sequence-id-105728

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  9. nes374tk/lacZ

    Plasmid
    #47615
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    374 bp from 2nd intron (comprising bp 1153 to 152…
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59894-plasmid-map-sequence-id-108252

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  18. pAAV-Ef1a-sRGECO (AAV8)

    Viral Prep
    #137125-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-sRGECO (#137125). In addition to the viral particles, you will also receive purified pAAV-Ef1a-sRGECO plasmid DNA. EF1a-driven expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV Retrograde)

    Viral Prep
    #26966-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-nEF-ChRmine-mScarlet (AAV8)

    Viral Prep
    #137158-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-ChRmine-mScarlet (#137158). In addition to the viral particles, you will also receive purified pAAV-nEF-ChRmine-mScarlet plasmid DNA. nEF-driven expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mScarlet
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 6081 - 6100 of 22037 results