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pCAG-OSF-KATNA1 R14A

Plasmid

#160052

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

R14A mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 V55D

Plasmid

#160053

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

V55D mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNA1 MIT residues 1-79, V55D

Plasmid

#180615

Purpose

Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-79, V55D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-4x mut_R

Plasmid

#147371

Purpose

Mammalian Expression of HsNot1iso1-4xmut

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-4xmut (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-K1208AH1212AK1218A_R

Plasmid

#147372

Purpose

Mammalian Expression of HsNot1iso1-K1208AH1212AK1218A

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-K1208AH1212AK1218A (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-K1208EH1212TK1218E_R

Plasmid

#147373

Purpose

Mammalian Expression of HsNot1iso1-K1208EH1212TK1218E

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-K1208EH1212TK1218E (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R

Plasmid

#147374

Purpose

Mammalian Expression of HsNot1iso1-P1209YP1257Y

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-P1209YP1257Y (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-P1257Y_R

Plasmid

#147375

Purpose

Mammalian Expression of HsNot1iso1-P1257Y

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-P1257Y (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP1a-Y36A_P

Plasmid

#147218

Purpose

Mammalian Expression of HsDCP1a-Y36A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsDCP1a-Y36A (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and G1716T compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-Y36A_P

Plasmid

#147221

Purpose

Mammalian Expression of HsDCP1a-Y36A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsDCP1a-Y36A (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1-1089_K

Plasmid

#146804

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_1085-1605_K

Plasmid

#146805

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1089_K

Plasmid

#146806

Purpose

Mammalian Expression of HsNot1iso1_1-1089

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1-1089 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1085-1605_K

Plasmid

#146807

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ZPBP-COMP5AP-AviTag-9xHis

Plasmid

#157311

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ZPBP (ZPBP Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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mRFP-PA-GFP-RhoAQ63L

Plasmid

#187282

Purpose

Express pmRFP-PAGFP-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

RhoA (RHOA Human)

Use

Tags

mRFP-PAGFP

Expression

Mammalian

Mutation

RhoA Q63L

Promoter

CMV, T7

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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ATX3-JD

Plasmid

#184247

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

Depositor

Sandra Macedo Ribeiro

Article

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 Josephin domain (ATXN3 Human)

Use

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Premature stop codon V183X

Promoter

T7 promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S2-Enz

Plasmid

#183768

Purpose

Expresses SHIP2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Use

Tags

6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

Expression

Bacterial

Mutation

includes aa K414 to aa V872 only

Promoter

T7lac

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst2-3xHA

Plasmid

#183541

Purpose

Express Dcst2-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 2 (Dcst2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin(N229A)-TEV-His6

Plasmid

#178077

Purpose

Insect cell expression of gamma-tubulin(N229A)-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin with an N229A point mutation (TUBG1 Human)

Use

Tags

TEV-His6

Expression

Insect

Mutation

Changed Asparagine 229 to Alanine

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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