Addgene

We narrowed to 16,077 results for: myc tag

Showing: 6061 - 6080 of 16077 results

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pETM33_Nsp16

Plasmid

#156482

Purpose

Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_N_NTD

Plasmid

#156483

Purpose

Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

N_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_N_CTD

Plasmid

#156484

Purpose

Bacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

N_CTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_Orf10

Plasmid

#156486

Purpose

Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Use

Tags

His tag (6x)

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pDM304_YFP-IqgC_mRFP-Raf1(RBD)

Plasmid

#128698

Purpose

simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

Inserts

iqgC (full length)
Raf1 kinase (aa 50-132)

Use

Dictyostelium discoideum expression vector

Tags

YFP and mRFP

Expression

Mutation

none

Promoter

Availability

Academic Institutions and Nonprofits only

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p2XStrep-ATIC

Plasmid

#125685

Purpose

expresses human ATIC protein with an N-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ATIC (ATIC Human)

Use

Tags

2X Strep-Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

Plasmid

#75122

Purpose

Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pcDNA3-SARS-CoV-2-S-RBD-8his

Plasmid

#145145

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Use

Tags

8his tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

Plasmid

#111398

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

Plasmid

#111399

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

Plasmid

#111400

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

Plasmid

#111451

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

Promoter

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-TEV-mCherry

Plasmid

#105800

Purpose

Concomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCherry

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT-sm

Plasmid

#214033

Purpose

Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

R456E, D459K, D476K, R479E

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216249

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgRANGAP.C1

Use

CRISPR; Endogenous tagging

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-F2A-T2A-Venus

Plasmid

#62621

Purpose

AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag

Depositor

Zheng-Xu Wang

Insert

MCS with F2A and T2A

Use

Adenoviral

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pANY4-pL18-ccdB

Plasmid

#164898

Purpose

Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.

Depositor

Yingfeng An

Insert

ccdB cassette

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/mKO2

Plasmid

#110353

Purpose

Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag

Depositor

Sandra Martha Gomes Dias

Insert

monomeric Kusabira Orange 2

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

gag

Availability

Academic Institutions and Nonprofits only

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pET28-H12TEV

Plasmid

#208635

Purpose

for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

Depositor

Quanfu Ma

Type

Empty backbone

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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