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Plasmid#131016PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH2ec
Plasmid#131018PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
KTK_048
Plasmid#180564PurposeEntry vector containing L3S1P00DepositorInsertL3S1P00_E1.4b
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_033
Plasmid#180545PurposeEntry vector containing LuxRDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CPL2g/pDONR221
Plasmid#172093PurposeGenomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221DepositorInsertCPL2 (CPL2 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationPromoterCPL2Available sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pColE1-AlphaOmega
Plasmid#165867PurposeTerminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj19b
Plasmid#173149PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19b (kcnj19b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj1a.1
Plasmid#173125PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj1a (kcnj1a.1 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11l
Plasmid#173140PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11l (kcnj11l Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-GMRWhite
Plasmid#165885PurposeBlasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW4
Plasmid#166710Purposeexpresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cellsDepositorInsertNEDD4L W576A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 576 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1
Plasmid#166707Purposeexpresses NEDD4L with inactivating mutation in first WW domain in mammalian cellsDepositorInsertNEDD4L W221A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E45: attB4-hr5-ie1p>-attB1r
Plasmid#162930PurposeGateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPVDepositorInsertAcMNPV ie1 promoter with hr5 enhancer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E06: attB5r-pA-polyhedrinp>-attB1r
Plasmid#162948PurposeGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertAcMNPV polyhedrin promoter with upstream polyA sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r
Plasmid#162965PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Halotag fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Plasmid#162964PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r
Plasmid#162957PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r
Plasmid#162955PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only