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Addgene
Showing: 6041 - 6060 of 16077 results

  1. Flag-M4-AR-W741C-S81A

    Plasmid
    #171248
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)
    Depositor
    Steven Balk
    Article
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    171248-plasmid-map-sequence-id-336016

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  2. Flag-M4-AR-W741C-S81D

    Plasmid
    #171249
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)
    Depositor
    Steven Balk
    Article
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    171249-plasmid-map-sequence-id-336007

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  3. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Article
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    170103-plasmid-map-sequence-id-332603

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  4. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

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  6. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid
    #153441
    Purpose
    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode B knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 140 and methionine 144 were mutated to a…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    153441-plasmid-map-sequence-id-306441

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Showing: 6041 - 6060 of 16077 results