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Plasmid#197500PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-RUNX1-WT
Plasmid#194574PurposeMammalian Expression of mEGFP-RUNX1 WT Fusion ProteinDepositorInsertRUNX1 (RUNX1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry-t2a-SOX2-p2a-KLF4
Plasmid#193296Purposeepisomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)DepositorUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-p2a-KLF4
Plasmid#193292Purposeepisomal hSK (expressing human SOX2 + KLF4)DepositorUseTagsExpressionMammalianMutationF2A of original pCXLE-SK vector was replaced with…PromoterCAGAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_XBB
Plasmid#194494Purposeexpressing SARS-CoV-2 XBB spike protein for pseudovirus productionDepositorInsertSpike (XBB) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationV83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…PromoterCMVAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_BQ.1.1
Plasmid#194493Purposeexpressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus productionDepositorInsertSpike (BQ.1.1) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationR346T, K444T, N460K and c-terminal 18aa del on th…PromoterCMVAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-45: TTN-mEGFP
Plasmid#114412PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertTTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-YAP1-shRNA1
Plasmid#176846Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)
Plasmid#197507PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CFLAR_WT_V5
Plasmid#82936PurposeGateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCFLAR (CFLAR Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_BA.4/5
Plasmid#186810Purposepseudovirus plasmid expressing BA.4 and BA.5 spike proteinDepositorInsert43740568 (S SARS-CoV-2)
UseTagsExpressionMammalianMutationHY69-70del, L452R, F486V, Q493R, on the basis of …PromoterCMVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-YAP1-shRNA2
Plasmid#176847Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh2 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro
Plasmid#176850Purposelentiviral vector for doxycycline-inducible YAP1 overexpressionDepositorInserthYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationfull length YAP1PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry-NPM1
Plasmid#194546PurposeBacterial Expression of 6xHis mCherry-NPM1 Fusion ProteinDepositorInsertNPM1 (Npm1 Mouse)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.L858R
Plasmid#82906PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationL858RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_LAGE3_WT_V5
Plasmid#83007PurposeGateway Donor vector containing LAGE3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertLAGE3 (LAGE3 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EBNA1BP2_WT_V5
Plasmid#82932PurposeGateway Donor vector containing EBNA1BP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertEBNA1BP2 (EBNA1BP2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ID2_WT_V5
Plasmid#82960PurposeGateway Donor vector containing ID2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertID2 (ID2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12D/sgKras/Cre
Plasmid#99851PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 S310F
Plasmid#40991DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationS310FPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only