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Plasmid#173135PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj9 (kcnj9 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10a
Plasmid#173137PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10a (kcnj10a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10b
Plasmid#173138PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10b (LOC100329607 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-FL
Plasmid#171602PurposeExpresses full length S. pombe Mdn1 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-D-iLight
Plasmid#170270PurposeExpression of iLight with 6xHis N-terminal tag in bacteria.DepositorInsertiLight
UseTags6xHis tagExpressionBacterialMutationSee CommentsPromoteraraBADAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 C415A
Plasmid#162633PurposeExpresses N-terminal 2x-Strep-tagged ORF68 C415A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C415 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 H452A
Plasmid#162634PurposeExpresses N-terminal 2x-Strep-tagged ORF68 H452A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid H452 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 C79A
Plasmid#162635PurposeExpresses N-terminal 2x-Strep-tagged ORF68 C79A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C79 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K435A
Plasmid#162636PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K435A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid K435 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 R443A
Plasmid#162637PurposeExpresses N-terminal 2x-Strep-tagged ORF68 R443A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid R443 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K450A/R451A
Plasmid#162638PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K450A/R451A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K450 and R451 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 R14A
Plasmid#162640PurposeExpresses N-terminal 2x-Strep-tagged ORF68 R14A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid R14 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K310A
Plasmid#162641PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K310A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid K310 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 R14A/K310A
Plasmid#162642PurposeExpresses N-terminal 2x-Strep-tagged ORF68 R14A/K310A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids R14 and K310 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K395A/K396A
Plasmid#162643PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K395A/K396A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K395 and K396 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-UL32 C128A
Plasmid#162644PurposeExpresses N-terminal 2x-Strep-tagged UL32 C128A in mammalian cellsDepositorInsertUL32
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C128 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-UL32 C502A
Plasmid#162645PurposeExpresses N-terminal 2x-Strep-tagged UL32 C502A in mammalian cellsDepositorInsertUL32
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C502 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-UL32 C308A/C309A
Plasmid#162646PurposeExpresses N-terminal 2x-Strep-tagged UL32 C308A/C309A in mammalian cellsDepositorInsertUL32
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids C308 and C309 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-UL32 C544A
Plasmid#162647PurposeExpresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cellsDepositorInsertUL32
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C544 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only