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Plasmid#67542PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCfB2198
Plasmid#67554PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2228
Plasmid#67540PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
BSP179
Plasmid#110915PurposeFor cloning expression cassettes by HR in yeast, Universal MosSCI compatibleDepositorTypeEmpty backboneUseSynthetic Biology; Yeast homologous recombination…TagsExpressionWormMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDH5
Plasmid#83775PurposeUseful for tagging yeast proteins with C-terminal YFP tag; his selectionDepositorInsertYFP
UseTagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…PromoterAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
JC172
Plasmid#104948PurposeMethanol inducing HSA-hGH fusion protein, and estrogen inducing TEV proteaseDepositorInsertHSA-hGH fusion protein and TEV protease
UseTagsExpressionMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2223
Plasmid#67544PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS1U-PKS12
Plasmid#70150PurposeFusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection markerDepositorInsertPKS12 (PKS12 Fusarium graminearum)
UseTagsExpressionYeastMutationPromoterCUP1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
JC031
Plasmid#104953PurposeMethanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing InterferonDepositorInserthGH, Interferon and G-CSF
UseTagsExpressionMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAH3
Plasmid#41827PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionYeastMutationPromoterTEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Thr
Plasmid#50721Purposeexpression of the human TPI Ile170Thr allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ThreoninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-pol30(K127R)-3'
Plasmid#99547PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationK127RPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)
Plasmid#14846DepositorInsertPHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)
Plasmid#14841DepositorInsertPHO5 promoter UASm1 (PHO5 Budding Yeast)
UseTagsVYFPExpressionYeastMutationUASm1 (AAGCTT was substituted for CACGTT). Unindu…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)
Plasmid#14844DepositorInsertPHO5 promoter TATA-C2 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
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300_pETcon_SARS2_FLip
Plasmid#222230Purposeyeast surface display of the SARS-CoV-2 FLip variant RBDDepositorInsertSARS-CoV-2 FLip RBD (S Budding Yeast, SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Q443P
Plasmid#216401PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutationDepositorInsertEfg1-ΔDBD-Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Glutamine 443 to Proline, and deleted ami…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisTagsExpressionYeastMutationPromoteryeast CYC-GAL and T7 promoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN100
Plasmid#205073PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 100 amino acidsPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only