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Showing: 5981 - 6000 of 14984 results

  1. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176460
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176460-plasmid-map-sequence-id-346305

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  2. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  3. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159676
    Purpose
    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159676-plasmid-map-sequence-id-314890

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  4. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159677
    Purpose
    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159677-plasmid-map-sequence-id-314891

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  5. ACE2 g1 + Cas9 (Lentiviral Prep)

    Viral Prep
    #153011-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from ACE2 g1 + Cas9 (#153011). In addition to the viral particles, you will also receive purified ACE2 g1 + Cas9 plasmid DNA. Lentiviral particles carrying a guide RNA targeting ACE2 and co-expressing Cas9 and TagBFP2.
    Depositor
    Jason Sheltzer
    Promoter
    Tags
    None
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  6. TMPRSS2 g1 + Cas9 (Lentiviral Prep)

    Viral Prep
    #153015-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from TMPRSS2 g1 + Cas9 (#153015). In addition to the viral particles, you will also receive purified TMPRSS2 g1 + Cas9 plasmid DNA. Lentiviral particles carrying a guide RNA targeting TMPRSS2 and co-expressing Cas9 and TagBFP2.
    Depositor
    Jason Sheltzer
    Promoter
    Tags
    None
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  7. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  8. pFG001

    Plasmid
    #62816
    Purpose
    Bacterial constitutive gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PN25
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    62816-plasmid-map-sequence-id-123824

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  9. pUC-H1-Esp3I-mU6-Kan

    Plasmid
    #213015
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)
    Depositor
    Stephanie Roessler
    Insert
    H1 promoter, mU6 promoter, gRNA scaffolds
    Use
    Tags
    Expression
    Mutation
    Promoter
    H1 promoter
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213015-plasmid-map-sequence-id-423154

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  10. pUC-tRNAGln-BbsI-sU6-Kan

    Plasmid
    #213016
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)
    Depositor
    Stephanie Roessler
    Insert
    tRNAGln promoter, sU6 promoter, gRNA scaffolds
    Use
    Tags
    Expression
    Mutation
    Promoter
    tRNAGln promoter
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213016-plasmid-map-sequence-id-423155

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  11. pALuWBB

    Plasmid
    #183202
    Purpose
    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA
    Depositor
    Mitch McVey
    Insert
    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    Use
    CRISPR; Recombines at attp landing site in fly ge…
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6-3
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    183202-plasmid-map-sequence-id-362157

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  12. LentiGuide-10X-CS1-3'-puro

    Plasmid
    #212011
    Purpose
    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)
    Depositor
    Danwei Huangfu
    Insert
    gRNA scaffold
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212011-plasmid-map-sequence-id-419682

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  13. pCJ1023

    Plasmid
    #228763
    Purpose
    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228763-plasmid-map-sequence-id-455783

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  14. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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  15. pUDP082

    Plasmid
    #103875
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103875-plasmid-map-sequence-id-199262

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  16. pUDP013

    Plasmid
    #103873
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103873-plasmid-map-sequence-id-199239

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  18. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

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  19. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128323-plasmid-map-sequence-id-255024

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  20. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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Showing: 5981 - 6000 of 14984 results