Addgene

We narrowed to 6,532 results for: 11

Showing: 5981 - 6000 of 6532 results

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Src(YF)-SsrA(Y7I)-mCer3

Plasmid

#220999

Purpose

this is microTag (Y7I mutation in SsrA)-Src(Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Y529F in Src, Y7I in SsrA

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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AA226

Plasmid

#216027

Purpose

Fragmid fragment: (Cas protein) Cas7-11

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas7-11_v1.1 [Di]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BL21(DE3) ΔserC

Bacterial Strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial Resistance

None

Availability

Academic Institutions and Nonprofits only
pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
HSC1-HS4-D4Z4c-GiP

Plasmid

#58541

Purpose

Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1-alpha

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2

Plasmid

#58681

Purpose

Expresses 3x Flag-tagged LGP2 in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

laboratory of genetics and physiology 2 (DHX58 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2-MI

Plasmid

#58682

Purpose

Expresses LGP2 with a mutation to motif I in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

LGP2-MI (DHX58 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Lysine 30 mutated to Alanine

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Cry2 mCherry PGK-1 in pcDNA3.1

Plasmid

#64214

Purpose

mCherry fused between Cry2 and PGK-1 to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
PGK1 (PGK1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Tags

His and roGFP2

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Use

Tags

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLPCX mito Grx1-roGFP2

Plasmid

#64977

Purpose

Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Promoter

Availability

Academic Institutions and Nonprofits only

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pMGF130

Plasmid

#96971

Purpose

S. pombe lem2 knockout cassette with hygro resistance gene

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lem2 (lem2 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

whole ORF deletion and replaced with hygro resist…

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

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pMGF172

Plasmid

#96998

Purpose

ish1 mCherry insertion cassette with ura4+ gene

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + ura4(…

Promoter

N/A

Availability

Academic Institutions and Nonprofits only

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LZRSpBMN-Z‐GFP‐Eps8

Plasmid

#74935

Purpose

Expresses mouse Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pUAST-HA, Pbl-A

Plasmid

#69792

Purpose

Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Depositor

Arno Muller

Article

van Impel et al Development. 2009 Mar;136(5):813-22. doi

Insert

Pebble - Drosophila guanine nucleotide exchange factor

Use

P element-based puast vector for gal4-regulated e…

Tags

HA tag

Expression

Insect

Mutation

Encodes Pbl (NP_729306.1) lacking last 9 amino ac…

Promoter

hsp70 promoter

Availability

Academic Institutions and Nonprofits only
pUAST-HA, Pbl-A DH domain

Plasmid

#69794

Purpose

Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Depositor

Arno Muller

Article

van Impel et al Development. 2009 Mar;136(5):813-22. doi

Insert

Pebble DH domain

Use

P element-based puast vector for gal4-regulated e…

Tags

HA-Tag

Expression

Insect

Mutation

Contains only the Pebble DH domain

Promoter

hsp70

Availability

Academic Institutions and Nonprofits only
pRR.1

Plasmid

#69995

Purpose

Mammalian expression of cytochrome p450 CYP2B6

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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