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Plasmid#192348PurposeLentiviral expression vector for an inducible shCLTC v2DepositorInsertshCLTC v2 (CLTC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCLTC v1 puro
Plasmid#192347PurposeLentiviral expression vector for an inducible shCLTC v1DepositorInsertshCLTC v1 (CLTC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-CLTC
Plasmid#227312PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.DepositorInsertsgRNA Targeting C-terminus of CLTC (CLTC Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-CHC17KDP
Plasmid#59799PurposeExpresses knockdown-proof human CHC17 tagged with GFP.DepositorInsertCHC17 (CLTC Human)
UseTagsGFPExpressionMammalianMutationSilent mutations in amino acids 61-65 to confer s…PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_M18
Plasmid#82357PurposeExpression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-CLTC
Plasmid#227313PurposeDonor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)DepositorInsertCLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-CHC(1-1074)-His6
Plasmid#100748PurposeBacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.DepositorInsertCLTC (CLTC Human)
UseTagsHis and MBPExpressionBacterialMutationResidues 1-1074PromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_M18
Plasmid#82356PurposeExpression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CLTC_Clathrin
Plasmid#109997PurposeProtein expression and purification of CLTC_ClathrinDepositorInsertCLTC_Clathrin (CLTC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC
Plasmid#214950PurposeBacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.DepositorInsertsVNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
UseTags6xHis, Amino half of venus BIFC fragment, Carboxy…ExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-CLTC
Plasmid#227314PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)DepositorInsertCLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-SRRD-mRuby3
Plasmid#214920Purposeinducible expression of SRRD tagged on C-terminus with mRuby3DepositorInsertSRRD (SRRD Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-noTag (in pLEX_307)
Plasmid#214919Purposeconstitutive expression of SRRD with no tag - used as control for SRRD-HA expressionDepositorInsertSRRD (SRRD Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-HA (in pLEX_307)
Plasmid#214918Purposeconstitutive expression of SRRD tagged with HADepositorInsertSRRD (SRRD Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-V5-APEX2 (in pLEX_307)
Plasmid#214921Purposeconstitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylationDepositorInsertSRRD (SRRD Human)
UseLentiviralTagsV5-APEX2ExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-c-Myc-Ubc-rtTA
Plasmid#198190PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthC-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC25A15_STOP
Plasmid#161133PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC25A15 (SLC25A15 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC25A15
Plasmid#131967PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A15 (SLC25A15 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
A24M04 mIgM heavy chain-pFEEKW
Plasmid#234809PurposeExpress membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1DepositorInsertA24M04 membrane IgM heavy chain (IGHM Human)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEEK promoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only