-
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-03
Plasmid#119982PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z03
Plasmid#120046PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P03
Plasmid#120014PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOCT4-Cyan-pac
Plasmid#160871PurposeMarmoset TargetingDepositorInsertmCerulean3
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
UseTagsmCerulean3ExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Src(WT)-SsrA(Y7I)-mCer3
Plasmid#220998PurposeThis is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminalDepositorInsertSrc, SsrA, mCerulean3 (Src Mouse)
UseTagsmCerulean3ExpressionMammalianMutationY7I in SsrAPromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Src(YF)-SsrA(Y7I)-mCer3
Plasmid#220999Purposethis is microTag (Y7I mutation in SsrA)-Src(Y529F) with mCerulean3 at C-terminalDepositorInsertSrc, SsrA, mCerulean3 (Src Mouse)
UseTagsmCerulean3ExpressionMammalianMutationY529F in Src, Y7I in SsrAPromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Src(KD/YF)-SsrA(Y7I)-mCer3
Plasmid#221000Purposethis is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminalDepositorInsertSrc, SsrA, mCerulean3 (Src Mouse)
UseTagsmCerulean3ExpressionMammalianMutationD388R/Y529F in Src, Y7I in SsrAPromoterpCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAF161
Plasmid#135926PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mCerulean3
UseTransposonTagsmCerulean3ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Control-Mosaic (SO274)
Plasmid#99751PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteinsDepositorInsertHIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA
Plasmid#216319PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Cerulean + splice acceptor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA
Plasmid#216318PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertsplit cerulean fluorescent protein + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TopBP1-mCer
Plasmid#211414Purposemammalian expression of human TopBP1 C-terminally fused to mCeruleanDepositorInsertTopBP1 (TOPBP1 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RecQL4-mCer
Plasmid#211402Purposemammalian expression of human RecQL4 C-terminally fused to mCeruleanDepositorInsertRecQL4 (RECQL4 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B CFP
Plasmid#198059PurposemCerulean fused to human H2B proteinDepositorInsertH2B
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCeruleanExpressionMutationPromotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C16orf59-mCer
Plasmid#211424Purposemammalian expression of human C16orf59 C-terminally fused to mCeruleanDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm5-mCer
Plasmid#211393Purposemammalian expression of human Mcm5 C-terminally fused to mCeruleanDepositorInsertMcm5 (MCM5 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdc45-mCer
Plasmid#211389Purposemammalian expression of human Cdc45 C-terminally fused to mCeruleanDepositorInsertCdc45 (CDC45 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53-mCer
Plasmid#211420Purposemammalian expression of human C17orf53 C-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only