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Showing: 581 - 600 of 2259 results

  1. pLKO_NWS.S.V5_mCherry-NLS

    Plasmid
    #178281
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.S.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178281-plasmid-map-sequence-id-353147

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  2. pLKO_NWS.Ollas.V5_mCherry-NLS

    Plasmid
    #178280
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.Ollas.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178280-plasmid-map-sequence-id-353145

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  3. pET-DEST42-dre4

    Plasmid
    #170440
    Purpose
    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.
    Depositor
    Joan Conaway
    Article
    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e
    Insert
    dre4 (dre4 Fly)
    Use
    Tags
    6xHis and V5
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-20
    Promoter
    T7
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170440-plasmid-map-sequence-id-338015

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  6. TFORF1482

    Plasmid
    #141828
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141828-plasmid-map-sequence-id-415124

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  14. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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  15. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid
    #182885
    Purpose
    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    stop codon introduced 2 amino acids downstream of…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182885-plasmid-map-sequence-id-367930

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  18. pDONR223-ZNF3_isoform2

    Plasmid
    #88280
    Purpose
    Donor Vector containing ZNF3 transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ZNF3 (ZNF3 Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 581 - 600 of 2259 results