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Showing: 41 - 60 of 8701 results

  6. pRSET a MatryoshCaMP6s

    Plasmid
    #100019
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    MatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    100019-plasmid-map-sequence-id-194295

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  7. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  8. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  13. p5'Entry-lck-5.5kb

    Plasmid
    #58890
    Purpose
    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    Use
    Gateway 5' entry clone
    Tags
    Expression
    Mutation
    mutated single nucleotide (T-->C, 13th nucleot…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Availability
    Academic Institutions and Nonprofits only
    58890-plasmid-map-sequence-id-91281

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  14. p5'Entry-lck-7.4kb

    Plasmid
    #58891
    Purpose
    provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
    Use
    Gateway 5' entry clone
    Tags
    Expression
    Mutation
    mutated single nucleotide (T-->C, 1874th nucle…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Availability
    Academic Institutions and Nonprofits only
    58891-plasmid-map-sequence-id-91398

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  15. Gal4-entry+NLS

    Plasmid
    #128013
    Purpose
    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Article
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    Gal4 DBD-SV40NLS-3x-Flag
    Use
    Gal4 entry vector with nls to generate tethering …
    Tags
    Gal4-DBD-SV40NLS-3xFlag
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128013-plasmid-map-sequence-id-251575

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Showing: 41 - 60 of 8701 results