Addgene

We narrowed to 4,474 results for: puc

Showing: 41 - 60 of 4474 results

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pUCXKT

Plasmid

#60682

Purpose

positive selection cloning and expression vector

Depositor

David Ackerley

Article

Prosser et al Biotechnol Lett. 2014 Sep 26.

Type

Empty backbone

Use

Positive selection e. coli expression vector

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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PUC9B71A

Plasmid

#44602

Depositor

Robert Oshima

Article

Singer et al J Biol Chem. 1986 Jan 15;261(2):538-47.

Insert

endoB (Krt18 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pUC12

Plasmid

#50002

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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SP_gRNA_pUC19_N_FancF_Left

Plasmid

#79892

Purpose

Human gRNA expression vector targeting FANCF (site 1 left)

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

FancF_Site_1_Left

Use

CRISPR

Tags

None

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19_TTE1564

Plasmid

#61000

Purpose

T7-transcription template for Tte mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_ABL1_e14a3

Plasmid

#190885

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e14a3 BCR::ABL1 fusion transcript
ABL1 cDNA (ABL1 Human)
GUSB cDNA (GUSB Human)
BCR (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e19a2

Plasmid

#190884

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e19a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e6a2

Plasmid

#190882

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts (e6a2)

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e6a2 cDNA
GUSB cDNA transcript (GUSB )
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e8a2

Plasmid

#190883

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e8a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-sg-1

Plasmid

#190607

Purpose

Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_BAS1509

Plasmid

#82354

Purpose

T7 in vitro transcription template for Bas mRNA

Depositor

Nils Walter

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;11

Insert

BAS1509 hypothetical protein

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_T790M_28bp

Plasmid

#114721

Purpose

28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790_28bp

Plasmid

#114720

Purpose

4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790_28bp

Plasmid

#114722

Purpose

28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790M_28bp

Plasmid

#114719

Purpose

4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC:FCP:ShBle:FCP:EGFP

Plasmid

#85987

Purpose

Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

Depositor

Thomas Mock

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Inserts

ShBle
EGFP

Use

Tags

Expression

Mutation

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19_extendedTTE1564

Plasmid

#60999

Purpose

T7-transcription template for Tte mRNA used in SiM-KARTS

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pUC120

Plasmid

#50350

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only