pUC19_4xT790M_28bp
(Plasmid
#114719)
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Purpose4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 2874
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFR
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SpeciesH. sapiens (human)
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Insert Size (bp)188
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Mutationc.2369C>T (Substitution, position 2369, C➞T); p.Threonine 790 to Methionine
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer tctgacacatgcagctcc
- 3′ sequencing primer caggaaacagctatgacc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Fragments contain 6 bp barcode TAGGAC at 3' end after digestion with MylI and ZraI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19_4xT790M_28bp was a gift from Nils Walter (Addgene plasmid # 114719 ; http://n2t.net/addgene:114719 ; RRID:Addgene_114719) -
For your References section:
Ultra-specific and Amplification-free Quantification of Mutant DNA by Single-molecule Kinetic Fingerprinting. Hayward SL, Lund PE, Kang Q, Johnson-Buck A, Tewari M, Walter NG. J Am Chem Soc. 2018 Aug 20. doi: 10.1021/jacs.8b06685. 10.1021/jacs.8b06685 PubMed 30125495