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Plasmid#187986Purposehuman p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationsilent mutation for resistance to sip62 #5PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 Double Mutation/Silent
Plasmid#187982PurposeK7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationD7A, D69A, silent mutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-LIR-mutant (LIRAAAA)
Plasmid#187981PurposePlasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationDDDW335-338AAAA (LIR mutant), silent mutation siR…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP5O_pCSdest
Plasmid#53812DepositorInsertATP5O (ATP5PO Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mGFP-AID
UseTagsAID(71-114)ExpressionYeastMutationPromoterADH1 and TEF1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mcherry p62 delta UBA silent
Plasmid#187984Purposemcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationLacking UBA domain (aa389-434) but it has the sil…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 delta PB1 silent
Plasmid#187985Purposehuman p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationLacking PB1 domain (aa1-102) with a silent mutati…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD 44 pGL4basic
Plasmid#78135Purposeluciferase reporter for miRNA activityDepositorInsertCD 44 (CD44 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCD44Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-ATP5O
Plasmid#139842PurposeLentiviral expression of human ATP5ODepositorInsertATP5O (ATP5PO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (fl)
Plasmid#137820Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull lengthPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44s (fl)
Plasmid#137812Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44s (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length, I186T and I253V on CD44PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v8-10 (fl)
Plasmid#137823Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v8-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull lengthPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6 (fl)
Plasmid#137816Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationR296K in the V6 region [Please see depositor comm…PromoterPGKAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v3 (fl)
Plasmid#137814Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v3 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length and N280D in the V3 regionPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v9 (fl)
Plasmid#137818Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v9 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length and mutation H48LPromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
UseTagsAID(71-114)ExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44s (-cyt)
Plasmid#137815Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44s (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic regionPromoterPGKAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (-cyt)
Plasmid#137821Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6-10 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic regionPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only