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Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-WDR5
Plasmid#200880PurposeMammalian expression of human WDR5 with N-terminal HaloTag fusionDepositorInsertHaloTag-WDR5 (WDR5 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
WDR5-HaloTag
Plasmid#200881PurposeMammalian expression of human WDR5 with C-terminal HaloTag fusionDepositorInsertWDR5-HaloTag (WDR5 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-HiBIT-WDR5
Plasmid#200883PurposeBacterial expression of N-terminal HiBIT-tagged human WDR5 proteinDepositorInsertHiBIT-WDR5 (WDR5 Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mouse sphingolipid metabolism knockout library
Pooled Library#206249PurposeThe sphingolipid metabolism library targets 82 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways.DepositorUseCRISPR and LentiviralAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTORC1 Focused library
Pooled Library#164084PurposeLentiviral CRISPR library targets human mTORC1 signaling pathwayDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
PsbA2-HMGS-HMGR-ATOB-NPTI
Plasmid#73338PurposeConstruct expressing the upper MVA pathway in Synechocystis PCC 6803DepositorInsertHMGS-HMGR-ATOB-NPTI
UseTagsExpressionMutationPromoterpsbA2Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUMV19
Plasmid#67161Purposeinducible operation of the mevalonate pathway in E. coliDepositorInsertthe mevalonate pathway genes
UseTagsExpressionBacterialMutationPromoterlacZAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Smad5
Plasmid#11744DepositorInsertSMAD5 (Smad5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Myc-Smad8
Plasmid#11745DepositorInsertSMAD8 (Smad9 Rat)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-PE4stem
Plasmid#208768PurposePE2max that co-express dnMLH1 and p53DDDepositorInsertPE4max with 2A-p53DD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AncBE4stem
Plasmid#208766PurposeAncBE4max that co-expresses UGI and p53DDDepositorInsertAncBE4max with 2A-UGI and 2A-p53DD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-ActRIIB
Plasmid#13501DepositorInsertActivin receptor IIB (Acvr2b Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCpSRb-4
Plasmid#13726DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHisExpressionBacterialMutationCircularly permuted SRβD210K183 with Precission p…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-ALK2 Q207D
Plasmid#11740DepositorInsertALK2 (ACVR1 Human)
UseTagsExpressionMammalianMutationQ207D: activated receptorPromoterAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-ActRIIA
Plasmid#11754DepositorInsertActivin receptor IIA (Acvr2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV5-ALK2 wt
Plasmid#11741DepositorInsertALK2 (ACVR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV5-ALK2 K235R
Plasmid#11755DepositorInsertALK2 (ACVR1 Human)
UseTagsExpressionMammalianMutationK235R: kinase deadPromoterAvailable sinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV5B-ALK4
Plasmid#11756DepositorInsertALK4 (ACVR1B Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits