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Plasmid#164856PurposeCre-inducible TIR1 expression vector for mouse H11 locusDepositorInsertOsTIR1
UseTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1amCherry-2A-OsTIR1-W
Plasmid#159296PurposeLentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) systemDepositorInsertOsTIR
UseLentiviralTagsExpressionMammalianMutationPromoterhuman EF1a promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK198 (GAL-OsTIR1)
Plasmid#140655PurposeGAL-OsTIR1(WT)DepositorInsertOsTIR1(WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG005 (Retron-TIR)
Plasmid#157883PurposeExpresses the retron-TIR defense systemDepositorInsertRetron-TIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(WT)-V5
Plasmid#158663PurposeTet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(WT) AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_NLS-Tir1-HA
Plasmid#117716PurposemRNA synthesis of NLS-TIR1-HA for injectionDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
UseTagsHA and NLSExpressionMammalianMutationcodon-optimized TIR1 for murine expressionPromoterSP6Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-OsTIR1(WT)
Plasmid#140729PurposeOsTIR1(WT)-P2A-mAID-EGFP-NESDepositorInsertOsTIR1(WT)-P2A-mAID-EGFP-NES
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1/Pur
Plasmid#171682Purposeconstitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertOsTIR1-MYC
UseTagsMYCExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-osTIR1-9myc
Plasmid#182043Purposein vitro transcription of osTIR1DepositorInsertosTIR1
UseIn vitro transcriptionTagsmycExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KJ101: pMVP (R4-R3) osTir1
Plasmid#121742PurposepMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc epitope tagExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-Tir
Plasmid#221326PurposeDox-inducible expression of Tir in mammalian cells by retroviral transductionDepositorInsertTir
UseRetroviralTagsHN-FLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK232 (CMV-OsTIR1-PURO)
Plasmid#72834PurposeExpresses OsTIR1 under the control of the CMV promoterDepositorInsertAAVS1-CMV-OsTIR1-PURO-AAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)
Plasmid#71720PurposeExpressing an mRuby-tagged TIR1 in the soma of C. elegansDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
UseTagsmRubyExpressionWormMutationcodon-optimized for expression in C. elegans; Two…Promotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIp204-PADH1-atTIR1-9myc
Plasmid#99532PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)DepositorInsertTIR1 (TIR1 Mustard Weed)
UseTags9xMycExpressionYeastMutationPromoterADH1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-ccvTIR1-NOSt
Plasmid#108546PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGExpressionMutationChanged F79 to GPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-osTIR1-IRES-GFP
Plasmid#110661PurposeLentiviral vector for stable expression of osTIR1 and selection with GFPDepositorInsertosTIR1
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PBS Actb-2A-TIR1-GFP
Plasmid#113832Purposetargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradationDepositorInsertosTIR1-GFP (NEWENTRY Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only