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Plasmid#120170PurposeExpresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
UseTagsHA and StreptavidinExpressionMammalianMutationTruncated KIF5B: 1-555PromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF1A*-strep
Plasmid#120165PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidinDepositorInsertkinesin family member 1A (Kif1a Mouse)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF1A: 1-405 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-HA
Plasmid#120171PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tagDepositorInsertkinesin family member C1 (KIFC1 Human)
UseTagsHA and StreptavidinExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF17*-strep
Plasmid#120167PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidinDepositorInsertkinesin family member 17 (KIF17 Human)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF17: 1-509 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF13A*-strep
Plasmid#120166PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidinDepositorInsertkinesin family member 13A (KIF13A Human)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF13A: 1-639 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF5A*-strep
Plasmid#120163PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidinDepositorInsertkinesin family member 5A (Kif5a Mouse)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF5A: 1-559 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mSA-EGFP-TM
Plasmid#39863DepositorInsertmonomeric streptavidin, EGFP, TM helix
UseTagsEGFP, FLAG, and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFsynW KDEL Hook
Plasmid#197277PurposeEncodes ER-targeted streptavidin-KDEL hook (RUSH system).DepositorInsertStreptavidin
UseLentiviralTagsFLAGExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaStrp
Plasmid#67508PurposeExpress the tRNAMet scaffold with the streptavidin aptamer tagDepositorInsertmodified tRNAMet with streptavidin aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDXNSM3
Plasmid#49028PurposeFX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), MYC, and str…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDXC3GMS
Plasmid#49031PurposeFX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptideDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), EGFP, MYC, a…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDXNSMG3
Plasmid#49029PurposeFX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage siteDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), EGFP, MYC, a…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDXC3MS
Plasmid#49030PurposeFX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptideDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), MYC, and str…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyTagsExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30b_OmpAT7Sav
Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[S1Apt]
Plasmid#68425PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct with S1 streptavidin aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneUseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-EGFP
Plasmid#65255Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertIi-Streptavidin and truncated Mannosidase II
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-EGFP
Plasmid#65252Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only