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Showing: 41 - 60 of 21839 results

  1. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.
    Depositor
    Bob Goldstein
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  2. Sybody Generation Toolbox

    Plasmid Kit
    #1000000160
    Purpose
    The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.
    Depositor
    Markus Seeger
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  3. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
    Depositor
    Irina Borodina
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.
    Depositor
    Helle Ulrich
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites
    Depositor
    Brigitte Gasser
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

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  8. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    John Dueber
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit
    #1000000191
    Purpose
    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.
    Depositor
    Arnold Driessen
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  10. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  11. Diatom uLoop Assembly Kit

    Plasmid Kit
    #1000000238
    Purpose
    The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.
    Depositor
    Christopher Dupont
    Available since
    Feb. 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  12. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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Showing: 41 - 60 of 21839 results