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Plasmid#200910Purpose3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsert3XHA_den1FnCas9_KRAB_eGFP
UseCRISPRTags3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFPExpressionMammalianMutationChanged Glutamic Acid to Arginine at 1369 positionPromoterCBHAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4a
Plasmid#83166PurposeGateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)DepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G13D
Plasmid#82808PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG13DPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.A59G
Plasmid#81662PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationA59GPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
XClone_Cas9_KRAB_T2A_BFP
Plasmid#141208PurposeTunable and temporal expression control of dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_PRKRA
Plasmid#106111PurposeInducible gene expression of FlagHA-PRKRADepositorInsertPRKRA (PRKRA Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_PRKRA
Plasmid#106110PurposeEntry vector for PRKRADepositorInsertPRKRA (PRKRA Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1
Plasmid#106114PurposeLentiviral expression of PRKRA delta1DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK (FLAG) and mycExpressionMutationAA 100-313PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23
Plasmid#106117PurposeLentiviral expression of PRKRA delta23DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK (FLAG) and mycExpressionMutationAA 1-127PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12A
Plasmid#81673PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12APromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12F
Plasmid#82807PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12FPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_KRAS_p.D33E
Plasmid#81659PurposeGateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationD33EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3
Plasmid#106115PurposeLentiviral expression of PRKRA delta3DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK and mycExpressionMutationTruncationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12S
Plasmid#81654PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12
Plasmid#106116PurposeLentiviral expression of PRKRA delta 12DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsExpressionMutationTruncationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12R
Plasmid#82804PurposeGateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.sgKras.9
Plasmid#91894PurposesgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.DepositorInsertKras sgRNA
UseLentiviralTagsExpressionMutationPromoterU6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G13V
Plasmid#82800PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG13VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_PRKRA
Plasmid#106109PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRADepositorInsertgRNA targeting PRKRA (PRKRA Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only