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Plasmid#113725PurposeExpresses the fluorescent protein mTagBFP2 in a lentiviral backboneDepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-N1
Plasmid#54595PurposeLocalization: N1 Cloning Vector, Excitation: 383, Emission: 448DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP-C1
Plasmid#54738PurposeLocalization: C1 Cloning Vector, Excitation: 387, Emission: 450DepositorTypeEmpty backboneUseTagsEBFPExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-ER-5
Plasmid#55294PurposeLocalization: Endoplasmic Reticulum, Excitation: 399, Emission: 456DepositorInsertER (CALR Human)
UseTagsmTagBFP2ExpressionMammalianMutationER Signal PeptidePromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
moxBFP
Plasmid#68064PurposeC-terminal fusion to oxBFPDepositorTypeEmpty backboneUseTagsmoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_IRES_mC_pGK_tBFP
Plasmid#79123PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch ReceptorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
TagBFP_A1aY1
Plasmid#158752PurposeLentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagBFP-T_A1aY1
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-C1
Plasmid#54665PurposeLocalization: C1 Cloning Vector, Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti6.2_TagBFP
Plasmid#113724PurposeExpresses the fluorescent protein TagBFP in a lentiviral backboneDepositorInsertTagBFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSExpressionMutationE174R, S542R, K548RPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45564PurposeExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequenceDepositorInsertsd14 eGFP donor
I-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationdeleted n' 15 amino acidsPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBFP2-C1
Plasmid#22880DepositorInsertSBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BFP
Plasmid#25891DepositorInsertBFP
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ERoxBFP
Plasmid#68126Purposemammalian expression of ER localized oxBFPDepositorInsertoxBFP
UseTagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
GalToxBFP
Plasmid#68073Purposemammalian expression of GC localized oxBFPDepositorInsertGalT signal anchor transmembrane domain
UseTagsmoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only