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Addgene

ERoxBFP
(Plasmid #68126)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68126 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    ER-mRFP
  • Backbone manufacturer
    Snapp et al. PMID 16617114, Addgene plasmid 62236
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    oxBFP
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • bovine prolactin signal sequence (N terminal on backbone)
    • KDEL ER retention sequence (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ERoxBFP was a gift from Erik Snapp (Addgene plasmid # 68126 ; http://n2t.net/addgene:68126 ; RRID:Addgene_68126)
  • For your References section:

    A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. 10.1038/ncomms8670 PubMed 26158227