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We narrowed to 607 results for: Anti-CRISPR

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Showing: 41 - 60 of 607 results

  3. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184835-plasmid-map-sequence-id-377028

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  4. pPlacPbuCas13b-gRNA-T-deGFP

    Plasmid
    #184840
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184840-plasmid-map-sequence-id-373961

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  5. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  6. pPbuCas13b-gRNA-NT

    Plasmid
    #184568
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184568-plasmid-map-sequence-id-374001

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  8. pPlacPbuCas13b-gRNA-T-MS2

    Plasmid
    #184839
    Purpose
    Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    184839-plasmid-map-sequence-id-373965

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  9. pPlacPbuCas13b-gRNA-NT

    Plasmid
    #184838
    Purpose
    Expression of single-spacer CRISPR array with a shortened non-targeting spacer for PbuCas13b and expression of PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Co-expression of single-spacer CRISPR array with a nontargeting spacer for PbuCas13b and PbuCas13b nuclease in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and lac promoter
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184838-plasmid-map-sequence-id-373981

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  10. pPgiCas13b-gRNA-1

    Plasmid
    #184834
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184834-plasmid-map-sequence-id-377030

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  12. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  13. pPbuCas13b-gRNA-1

    Plasmid
    #184567
    Purpose
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Depositor
    Chase Beisel
    Article
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184567-plasmid-map-sequence-id-374002

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  15. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61272-plasmid-map-sequence-id-99585

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  16. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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Showing: 41 - 60 of 607 results