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Showing: 41 - 60 of 131 results

  1. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194887
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194887-plasmid-map-sequence-id-382813

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  2. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

    Plasmid
    #60394
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. SERPINA-bio-His

    Plasmid
    #52182
    Purpose
    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SERPINA (SERPINA1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52182-plasmid-map-sequence-id-80945

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  18. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Article
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  19. pATZ-BFP-HA-GFP11

    Plasmid
    #177721
    Purpose
    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    alpha-1-antitrypsin (SERPINA1 Human)
    Use
    Tags
    GFP11, HA, and TagBFP
    Expression
    Bacterial and Mammalian
    Mutation
    E342K (ATZ mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177721-plasmid-map-sequence-id-352621

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  20. pQCXIP-SHMY-A1Pi

    Plasmid
    #182648
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    A1Pi (SERPINA1 Human)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182648-plasmid-map-sequence-id-358630

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Showing: 41 - 60 of 131 results