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TagBFP_A1aY1

Plasmid

#158752

Purpose

Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagBFP-T_A1aY1

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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BCL2A1A

Plasmid

#42399

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BCL2A1A (BCL2A1 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
6x-His_A1aY1_GFP

Plasmid

#158753

Purpose

Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_A1aY1_GFP

Use

Tags

6x-Histidine tag, GFP, T7 leader sequence, and Th…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBA1A

Plasmid

#111695

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBA1A (TUBA1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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A1ACR1_EYFP_pcDNA3.1

Plasmid

#161025

Purpose

Expresses strongly red-shifted A1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

A1ACR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CACNA1A_Halo_N_allele

Plasmid

#178152

Purpose

Donor vector for endogenous tagging of human CACNA1A at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (CACNA1A Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFUS_A1A

Plasmid

#43848

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFUS2_a1a

Plasmid

#50680

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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EFNA1A

Plasmid

#25358

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

efna1a.017.171 (EPHA1 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pStA1AZ

Plasmid

#114162

Purpose

Start-Stop Assembly Level 1AZ plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA1AB

Plasmid

#114163

Purpose

Start-Stop Assembly Level 1AB plasmid

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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PL08008A1A06

Plasmid

#44671

Depositor

Phillip Newmark

Article

Chong et al BMC Dev Biol. 2011 Nov 10;11:69.

Insert

EST Contig 5859

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
PL08007A1A01

Plasmid

#44684

Depositor

Phillip Newmark

Article

Chong et al BMC Dev Biol. 2011 Nov 10;11:69.

Insert

Smed-tkn-2

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-alpha1AR

Plasmid

#106064

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-alpha1AR

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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ADRA1A-Tango

Plasmid

#66213

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADRA1A (ADRA1A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG2IG-Hmga1a

Plasmid

#122293

Purpose

Overexpression for mouse Hmga1a

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Hmga1a (Hmga1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA1a-RFP

Plasmid

#35334

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBS ZfHoxa1a

Plasmid

#27513

Depositor

Victoria Prince

Insert

Hoxa1a (hoxa1a Zebrafish)

Use

Riboprobe production

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-kcnma1a

Plasmid

#164951

Purpose

Zebrafish kcnma1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1a (kcnma1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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