1. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

   Plasmid

   #208039

   Purpose

   Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa; 3MAS) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, N-terminal Split-TurboID

   Expression

   Mutation

   3MAS mutant: K65R, K160R and K490R; mutated SIM

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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