1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. LentiCRISPRv2-hyg2-dEreg

   Plasmid

   #173843

   Purpose

   A knockout vector for the dog Ereg.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170812

   Purpose

   Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LentiCRISPRv2bleo-Nectin-2

   Plasmid

   #170295

   Purpose

   A knock-out vector for the mouse Nectin-2

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   A gRNA targeting the mouse Nectin-2 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiCRISPRv2bleo-Necl-5

   Plasmid

   #170294

   Purpose

   A knock-out vector for the mouse Necl-5

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   A gRNA targeting the mouse Necl-5 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MS2-adRNA (5'GAC)

   Plasmid

   #170127

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(5'GAC)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. MS2-adRNA (3'GAC)

   Plasmid

   #170129

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(3'GAC)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. LcV2-Hygro

   Plasmid

   #91977

   Purpose

   LentiCRISPR v2 with Hygro cloned in place of Puro

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgPBRM1-1- LentiCRISPRv2

   Plasmid

   #107403

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgPBRM1-2- LentiCRISPRv2

   Plasmid

   #107404

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiV2-EF1α-nCas9/RT

    Plasmid

    #210188

    Purpose

    Expression of nCas9/RT-P2A-Puro

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    nCas9/RT-P2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiCRISPRv2puro-Tbxas1

    Plasmid

    #170305

    Purpose

    A knock-out vector for the mouse Tbxas1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxas1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR-v2-FE-PuroR

    Plasmid

    #186746

    Purpose

    Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiCRISPRv2puro-Tbxa2r

    Plasmid

    #170304

    Purpose

    A knock-out vector for the mouse Tbxa2r

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxa2r gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCRISPRv2puro-Ptgs2_B6

    Plasmid

    #170300

    Purpose

    A knock-out vector for the mouse Ptgs2 in C57BL6

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiCRISPRv2puro-Ptger1

    Plasmid

    #170306

    Purpose

    A knock-out vector for the mouse Ptger1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptger1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPRv2puro-Ptgs2_Balbc

    Plasmid

    #170301

    Purpose

    A knock-out vector for the mouse Ptgs2 in Balb/c

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiCRISPRv2puro-Gnaq

    Plasmid

    #170302

    Purpose

    A knock-out vector for the mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Gnaq gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPRv2-neo-dTgfa

    Plasmid

    #173842

    Purpose

    A knockout vector for the dog Tgfa.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiCRISPRv2-puro-dEgfr

    Plasmid

    #173844

    Purpose

    A knockout vector for the dog Egfr.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiCRISPRv2hyg-Ptgs1

    Plasmid

    #170299

    Purpose

    A knock-out vector for the mouse Ptgs1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptgs1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge