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pM115: CasRx control presgRNA

Plasmid

#166867

Purpose

U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

non-targeting presgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-MCS no-pA

Plasmid

#80825

Purpose

P2A followed by multiple cloning site without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-MCS no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR11 mSTIM2 myc

Plasmid

#17734

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM2 (Stim2 Mouse)

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pF3BGX

Plasmid

#138387

Purpose

Empty RMCE vector (phiC31 attB/loxP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mScarlet-I

Plasmid

#138391

Purpose

RMCE vector for generating C-terminal mScarlet-I fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mScarlet-I

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR2-R2-PBR-L4

Plasmid

#141017

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-PBL-R1

Plasmid

#141016

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA2.1

Plasmid

#64117

Purpose

Vector for expression of St1 sgRNA2.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA2.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pNP151

Plasmid

#106359

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.

Depositor

Jonathan Ewbank

Article

Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

Insert

col-62p_lifeact_mkate2_3'nmy-2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-SSFV-dCas9-GFP

Plasmid

#64105

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

SSFV

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAG373-mito-redFAST

Plasmid

#160358

Purpose

Expresses mito-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

mito-redFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-P2rP4

Plasmid

#141015

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-LynEGFP

Plasmid

#133923

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL4

Plasmid

#138392

Purpose

RMCE vector for generating C-terminal T2A-GAL4 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFU-no toxin-PE

Plasmid

#24149

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

PDGF-receptor-transmembrane domain (PDGFRB Aequorea victoria, Human)

Use

Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2010

Availability

Academic Institutions and Nonprofits only
pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pICSL50016

Plasmid

#50315

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.

Use

Plant expression

Tags

Expression

Mutation

Codon-optimised for A. thaliana. BsaI/BbsI sites …

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-4u

Plasmid

#13553

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T163A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T163A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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myc-Orai1 E106A pHAGE

Plasmid

#22752

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 E106A (ORAI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E106A

Promoter

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only