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Plasmid#80603PurposeExpresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus endDepositorInsertEB1 (MAPRE1 Human)
UseTagsmCherryExpressionMammalianMutationTruncated C-terminus; EB1 coding sequence ends at…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
UseTagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha
Plasmid#58256Purposebacterial expression of human PITP alphaDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DCX-EGFP
Plasmid#80599PurposepLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)DepositorInsertDoublecortin (DCX Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti F9-TetR-EGFP-IRES-PuroR
Plasmid#117049Purposelentiviral vector for expression of TetR-EGFP, for visualization of TetO repeatsDepositorInsertF9-TetR-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterF9 promoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Pou4f3
Plasmid#158530PurposeExpresses human Pou4f3 in mammalian cellsDepositorInsertPOU Class 4 Homeobox 3 (POU4F3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_empty
Plasmid#203811PurposePlasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo1-G3BP1_pTRE-G418
Plasmid#202015Purposeexpresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-G3BP1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS614_pTetR-P2A-BFPnls/sgNS
Plasmid#108650PurposeNon-specific Spy sgRNA under U6 promoterDepositorInsertsNon-specific sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mBlueberry2-P2A-EGFP
Plasmid#184937PurposeExpresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cellsDepositorInsertmBlueberry2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…TagsExpressionPlantMutationPromoterE12-omegaAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LZRS-hNEDD9_IresGFP
Plasmid#21962DepositorInsertNEDD9 (NEDD9 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 wt (Nigg RG6)
Plasmid#41160DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-PSD95.FingR-eGFP-CCR5TC
Plasmid#125691PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only