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Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBER
Plasmid#62662PurposeDonor vector for tdTomato knock-in via landing padDepositorInsertstdTomato
hygromycin
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGex2t SadBs
Plasmid#66882PurposeBacterial expression vector encoding N-terminally GST-tagged short isoform of human SadBDepositorInsertSadB (BRSK1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1CH2AB
Plasmid#13475DepositorInsertMKP-1CH2AB (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationContains aa 1-136PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 short UTR
Plasmid#21639DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK2 del174-177
Plasmid#118297PurposeExpression of Rat ERK2DepositorInsertERK2 del174-177 (Mapk1 Rat)
UseTagsGFPExpressionMammalianMutationdeletion 174aa-177aaPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOCC110
Plasmid#118905Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)DepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and LPETGgExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC219
Plasmid#118908Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tagDepositorInsertNcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
UseTagsYBBR tag, HIS10, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D444-630
Plasmid#19840DepositorInsertMKL1 D444-630 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 444-630PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0011
Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRTagsExpressionMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5
Plasmid#52540PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 rc
Plasmid#21643DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S70A
Plasmid#13620DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 70 to AlaPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pBGO
Plasmid#62671PurposeDonor vector for mFTH1 knock-in via landing padDepositorInsertFTH1
UseCre/LoxTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-mEKLF
Plasmid#67838PurposeExpression of murine EKLF (nucleotides 71-1215) driven by SV40 promoterDepositorInsertEKLF (Klf1 Mouse)
UseTagsnoneExpressionMammalianMutationPromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -