Addgene

We narrowed to 14,735 results for: LEA

Showing: 5901 - 5920 of 14735 results

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PD-1-miSFIT-6-C, 16-C

Plasmid

#124681

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-TAF15N-mCherry-sspB

Plasmid

#122436

Purpose

Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

mCherry and sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHBS1449 SED1-Lenti

Plasmid

#180824

Purpose

SED1 biosensor for stable integration and expression in mammalian cells

Depositor

Rajat Rohatgi

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 osmosensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al bioRxiv 2022.10.04.510646

Insert

alpha synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-4x

Plasmid

#124678

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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Fv-hCaspase 8 (C/A)-2A-GFP

Plasmid

#82713

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8 (C/A)-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-Ce67

Plasmid

#124686

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-Phi29(+exo) (LM2759)

Plasmid

#208964

Purpose

Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29(+exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Dre

Plasmid

#55633

Purpose

Expresses Dre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Inserts

mCherry
Dre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pAGT6471

Plasmid

#219428

Purpose

Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

AGGT_NLS-SpCas9i-NLS(no stop)_TTCG

Use

Moclo compatible level 0 module

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCMV10x-sct-pp65-b2m-HLA-A0201

Plasmid

#196513

Purpose

Express single-chain pMHC with sequencing tag for 10x genomics

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C, 10xgenomics compatible tag

Promoter

CMV

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO EYFP

Plasmid

#55641

Purpose

Flp-Dependent EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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alpha Synuclein-VC

Plasmid

#89471

Purpose

expresses human alpha Synuclein fused to the C-terminal part of Venus

Depositor

Tiago Outeiro, Bradley Hyman

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Use

Tags

C-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-CCO-IRES-Puromycin

Plasmid

#134861

Purpose

Expresses EGFP-tagged inner mitochondrial membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome c oxidase subunit VIII (COX8A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.9

Plasmid

#102918

Purpose

expresses ABE7.9 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.9

Use

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-SpRY-NLS-6xHis (gbr2101)

Plasmid

#181743

Purpose

bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

bacterial codon optimized SpRY

Use

In vitro transcription; t7 promoter

Tags

BPNLS(SV40)-6xHis and MKIEE MBP tag

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

T7

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-10

Plasmid

#72348

Purpose

Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Use

Tags

6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

Expression

Mammalian

Mutation

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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