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CMYA5T2/pENTR3C

Plasmid

#97409

Purpose

CMYA5 (NM_153610, nt 12205 to 12279) T allele (rs10043986) in pENTR3C, Gateway Entry vector

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Unspecified

Tags

Expression

Mutation

rs10043986 (NM_153610.4:c.12188C>T)

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac1-OSF-TRIMCyp (owl monkey)

Plasmid

#79035

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-mRuby2 L1-R5

Plasmid

#84389

Purpose

Multisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Gateway

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBW301ParsR-Amp30T

Plasmid

#78653

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Ubl4A K48R

Plasmid

#61809

Purpose

expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K48R

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pREG(R1-R4) #BGV059

Plasmid

#48991

Purpose

Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

Plasmid

#52898

Purpose

YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ypd*1-L-IFP-F[2]

Use

Tags

L-IFP-F[2]

Expression

Yeast

Mutation

Changed Tryptophane 80 to Alanine

Promoter

GAL1

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
pENTR4_EWSR1_C2G_R650G

Plasmid

#26371

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Tags

Expression

Mutation

R650G

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2_BirA_NRF2

Plasmid

#136524

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-ECFP

Plasmid

#48353

Purpose

Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

ECFP

Use

Gateway entry vector

Tags

Expression

Mutation

Contains a stop codon.

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xSTOP-lox (JDW 869)

Plasmid

#224521

Purpose

A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LoxP-3xStop-LoxP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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amajLime chromoprotein

Plasmid

#117843

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amajLime

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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aeBlue chromoprotein

Plasmid

#117846

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, aeBlue

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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asPink chromoprotein

Plasmid

#117838

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, asPink

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein

Plasmid

#117848

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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spisPink chromoprotein

Plasmid

#117839

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, spisPink

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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gfasPurple chromoprotein

Plasmid

#117849

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, gfasPurple

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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meffBlue chromoprotein

Plasmid

#117845

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffBlue

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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