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Plasmid#127652PurposeKnock-out of human NONODepositorInsertNONO sgRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-Nono-sh1
Plasmid#127650PurposeKnock-down of human NONODepositorInsertNONO shRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTwist-WIV1-CoV Δ18
Plasmid#164439PurposeEncodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertWIV1-CoV Spike truncated to remove 18 amino acids
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-Nono-sh5
Plasmid#127651PurposeKnock-down of human NONODepositorInsertNONO shRNA (NONO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FcD265A-FLAG
Plasmid#65420PurposeMammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 productionDepositorInsertFcD265A-FLAG
UseTagsFLAGExpressionMammalianMutationD265APromoterModified CMV PromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-JAK1Gly590Arg
Plasmid#174573PurposeHuman FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)DepositorInsertJAK1Gly590Arg
UseTagsFLAGExpressionMammalianMutationGly590ArgPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX-Myc-hTIM3
Plasmid#110893PurposeExpression of Myc-tagged hTIM3 at the cell surfaceDepositorInsertHepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)
UseRetroviralTagsMycExpressionMutationPromoterCMVAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-aaAFB2
Plasmid#207839PurposeInducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsatAFB2
mCherry
miniIAA7
VHH-GFP4
UseTags3X FLAG-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TGMP.shKras.1422
Plasmid#59913PurposeTet on shKras for conditional Kras knockdownDepositorInsertKras (Kras Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLHCX-mPM20D1-flag
Plasmid#84566Purposemouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selectionDepositorInsertpeptidase M20 domain containing 1 (Pm20d1 Mouse)
UseRetroviralTags6xHis and FlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-cka
Plasmid#195067PurposeExpresses FLAG-tagged Drosophila cka proteinDepositorInsertConnector of kinase to AP-1 (Cka Fly)
UseTagsExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA-NONO-1
Plasmid#127654PurposeKnock-out of human NONO (guide only)DepositorInsertNONO sgRNA (NONO Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
KHDRBS3_KH_pGEX
Plasmid#135133PurposeRecombinant expression of KHDRBS3 with dual-affinity tagDepositorInsertKHDRBS3 (KHDRBS3 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
TRPML1-mCherry
Plasmid#135189PurposeExpresses TRPML1 with C-terminus mCherry tag in mammalian cells.DepositorInsertTRPML1 (Mcoln1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-STAT-CA
Plasmid#195576PurposeExpresses STAT3 constitutively active mutantDepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAAVTagsExpressionMammalianMutationchanged Alanine 662 to Cysteine and Asparagine 66…PromoterCMV enhancer and promoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA pcDNA3
Plasmid#206069Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain IIDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W
Plasmid#105023PurposeLentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFPDepositorInsertNelfb (Cobra1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W
Plasmid#105024PurposeLentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFPDepositorInsertNelfb (Cobra1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant
Plasmid#215026PurposeFirefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutatedDepositorInsertMinimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
UseLuciferaseTagsExpressionMutation4X E-box mutations within Coordinator motifsPromoterSV40Available sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only