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We narrowed to 14,735 results for: LEA

Showing: 5861 - 5880 of 14735 results

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AAVi U6_gRNA CMV_SadCas9-KRAB

Plasmid

#214609

Purpose

All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

Depositor

Ralf Gilsbach

Article

Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

Inserts

gRNA
SadCas9

Use

AAV

Tags

NP NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV and U6

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.10

Plasmid

#102919

Purpose

expresses ABE7.10 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.10

Use

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-DYRK1A

Plasmid

#101770

Purpose

Mammalian expression of DYRK1A fusion protein

Depositor

Michael Huen

Article

Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

Insert

DYRK1A (DYRK1A Human)

Use

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-ABE8e(V106W)

Plasmid

#198533

Purpose

Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

Plasmid

#133027

Purpose

Expresses EGFP-tagged late endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB7A (RAB7A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

Plasmid

#176280

Purpose

Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBac-P2A-EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin I

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-NurRE3

Plasmid

#208253

Purpose

pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

Depositor

Douglas Kojetin

Article

Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

Insert

3X Nur response element LUC

Use

Tags

No

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

Plasmid

#176281

Purpose

Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBacMUT-P2A-EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

E191K

Promoter

human Synapsin I

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-ABE8e(V106W)

Plasmid

#198547

Purpose

Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

Plasmid

#121966

Purpose

Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

scFv-GCN4

Use

Lentiviral

Tags

superfold GFP-GB1-NLS-iLID

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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SpCas9-YE1-BE4max

Plasmid

#198546

Purpose

Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-YE1-BE4max

Plasmid

#198535

Purpose

Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Fv-hCaspase 8-2A-GFP

Plasmid

#82712

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

LTR

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_Halo_GluA2

Plasmid

#170454

Purpose

Expresses halo tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Article

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

Plasmid

#61628

Purpose

Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

Depositor

Dolf Weijers

Article

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

ntdTomato
n2xEGFP

Use

Tags

Expression

Plant

Mutation

2 copies of EGFP with N-terminal SV40 NLS

Promoter

Auxin-dependent DR5 promoter containing 9 reverse…

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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