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Plasmid#139070PurposeLow phosphate inducible promoter amnp for GFP expressionDepositorInsertamnp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-mipAp-GFPuv
Plasmid#139071PurposeLow phosphate inducible promoter mipAp for GFP expressionDepositorInsertmipAp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoEp-GFPuv
Plasmid#139074PurposeLow phosphate inducible promoter phoEp for GFP expressionDepositorInsertphoEp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoHp1-GFPuv
Plasmid#139075PurposeLow phosphate inducible promoter phoHp1 for GFP expressionDepositorInsertphoHp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoUp-GFPuv
Plasmid#139076PurposeLow phosphate inducible promoter phoUp for GFP expressionDepositorInsertphoUp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-pstSp-GFPuv
Plasmid#139077PurposeLow phosphate inducible promoter pstSp for GFP expressionDepositorInsertpstSp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140168PurposeExpresses a regulatable secretory cargo for Tetrahymena thermophilaDepositorInsertssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Orange)
Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Crimson)
Plasmid#140156PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Plasmid#140155PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_015
Plasmid#123991PurposeEncodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthROSA26 sg4
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8_005
Plasmid#123885PurposeEncodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK systemDepositorInsertF1-KanR-ColE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_021
Plasmid#123936PurposeEncodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK systemDepositorInsertTUL3R4 spacer
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS160
Plasmid#134238PurposeLentiviral plasmid expressing sgRNA against HJURPDepositorInsertHuman HJURP sgRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM328
Plasmid#134243PurposeMammalian expression construct expressing sgRNA against PLK1DepositorInsertgRNA targeting PLK1 3'
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-pT7-sfGFP_RED20
Plasmid#135173PurposeExpresses sfGFP recoded in the RED20 genetic code off the T7 promoterDepositorInsertsfGFP_RED20
UseSynthetic BiologyTagsExpressionMutationRecoded ORF of sfGFP to only use one codon per am…PromotertaatacgactcactataAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only